Catalog Number - 68508-15P-2

Cogent RP C8™ HPLC Columns are designed for reliable reversed-phase chromatography using a traditional octyl (C8) bonded stationary phase. Manufactured with ultra-high purity silica and fully double end capped bonding technology, these columns provide reproducible retention, excellent peak shape, and long column life. The RP C8™ phase is a USP L7 equivalent chemistry and is commonly used as an alternative for methods developed on similar C8 phases. Compared to C18 columns, RP C8™ generally provides lower hydrophobic retention while maintaining excellent selectivity for many neutral and non-polar compounds.

68508-15P-2 is packed with 5 µm RP C8™ particles featuring a 100 Å pore size in a 2.1 mm ID x 150 mm length format. The 150 mm column length provides increased resolving power and peak capacity for more complex separations while maintaining the low solvent consumption benefits of a narrow-bore column. The RP C8™ stationary phase is particularly useful for hydrophobic compounds, neutral analytes, and long-chain fatty acids where somewhat lower retention than a comparable C18 column is desired. The 5 µm particle size provides low backpressure and broad compatibility with conventional HPLC and LC-MS systems.

This column is frequently selected for LC-MS applications when maximum analytical resolution is required. The 2.1 mm ID format minimizes mobile phase consumption and is highly compatible with mass spectrometric detection. Optimum performance is typically achieved at approximately 0.2-0.3 mL/min. Standard HPLC systems used with this column must be capable of delivering accurate and reproducible low flow rates, and low-volume detector flow cells are recommended to minimize extra-column dispersion and maintain column efficiency.

Key Benefits

• RP C8™ stationary phase

• USP L7 equivalent chemistry

• Octyl (C8) bonded phase

• Fully double end capped

• Ultra-high purity silica support

• 100 Å pore size

• High resolving power

• Lower retention than C18 phases

• Reduced solvent consumption

• Excellent for LC-MS applications

Typical Applications

• LC-MS analysis

• Reversed-phase HPLC

• USP L7 method substitution

• Method development

• Pharmaceutical analysis

• Long-chain fatty acid analysis

• Hydrophobic compound separations

• Neutral compound analysis

• Bioanalytical testing

• High-resolution analytical separations

Specifications

• Product Type: HPLC Column

• Stationary Phase: RP C8™

• USP Classification: L7

• Chromatography Mode: Reversed Phase

• Bonded Phase: Octyl (C8)

• Endcapping: Fully Double End Capped

• Particle Size: 5 µm

• Pore Size: 100 Å

• Column Dimensions: 2.1 mm ID x 150 mm

• Recommended Flow Rate: 0.2-0.3 mL/min

• Column Hardware: Stainless Steel

• Brand: Cogent

Important Notes

• Designed for high-resolution reversed-phase HPLC and LC-MS separations

• Provides lower retention than comparable C18 columns

• Suitable for hydrophobic and moderately non-polar compounds

• Excellent choice for long-chain fatty acid separations

• Frequently selected for USP L7 method replacement and transfer

• 150 mm length provides greater resolving power and peak capacity than shorter columns

• 5 µm particles provide low backpressure and broad instrument compatibility

• Requires an HPLC system capable of precise and reliable low flow rate delivery

• Low-volume detector flow cells are recommended to maximize chromatographic efficiency

• Manufactured using ultra-high purity silica and fully double end capped bonding technology